Aktivitas Antioksidan dan Penghambatan Enzim α-amilase Ekstrak Air Kombinasi Daun Papasan (Coccinia grandis [L.]) dan Daun Sembung (Blumea balsamifera [L.] DC)

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Atikawati Atikawati
I Gusti Ayu Wita Kusumawati
I Made Wisnu Adhi Putra
Ida Bagus Agung Yogeswara

Abstract

ABSTRAK
Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dan penghambatan α-amilase pada kombinasi ekstrak air daun papasan dan daun sembung. Ekstrak dibuat dengan cara menyeduh serbuk daun papasan dan daun sembung dengan berbagai rasio (1:3, 1:1, dan 3:1). Penyeduhan dilakukan menggunakan 100 ml air mendidih (100˚C) selama 5 menit. Hasil penelitian menunjukkan bahwa aktivitas antioksidan dalam menangkal radikal bebas DPPH tertinggi terdapat pada sampel kombinasi 1:3 yang dinyatakan dengan IC50 sebesar 1,58±0,06 mg/ml danaktivitas penghambatan α-amilase tertinggi juga terdapat pada sampel kombinasi rasio 1:3 dengan nilai IC50 sebesar 1,29±0,02 mg/ml. Secara umum, semua kombinasi berpotensi sebagai minuman fungsional antidiabetes.
Kata kunci: Daun papasan, daun sembung, aktivitas antioksidan, aktivitas penghambatan α-amilase, ekstrak air.

ABSTRACT
This study aims to determine the antioxidant and inhibition of α-amylase activities of the combination of Coccinia grandis [L.] and Blumea balsamifera [L.] DC water extracs. The extracts wass made by decoctingthe leaves powder in various ratios (1: 3, 1: 1, and 3: 1). Decoction process was done using 100 ml of infuced water (100°C) for 5 minutes. The results showed that thethe highest antioxidant activity in counteracting free radicals DPPH was found in combination of 1:3 expressed by IC50 of 1.58±0.06 mg/ml and the highest α-amylase inhibitory activity was also found in combination of 1:3sample with IC50 values of 1.29±0.02 mg/ml. In general, all combinations have potential to be antidiabetic functional drinks.
Keywords: Coccinia grandis [L.], Blumea balsamifera [L.] DC, antioxidant and inhibition of α-amylase, water extract.

Article Details

How to Cite
Atikawati, A., Kusumawati, I. G. A. W., Putra, I. M. W. A., & Yogeswara, I. B. A. (2019). Aktivitas Antioksidan dan Penghambatan Enzim α-amilase Ekstrak Air Kombinasi Daun Papasan (Coccinia grandis [L.]) dan Daun Sembung (Blumea balsamifera [L.] DC). Jurnal Media Sains, 3(2). Retrieved from http://jurnal.undhirabali.ac.id/index.php/jms/article/view/911
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