Analysis of Artificial Sweetener Levels in Beras Kencur Herbal Drinks Marketed at Gading Rejo Market Using U-HPLC

Authors

  • Muhammad Zaky Malik Universitas Aisyah Pringsewu, Lampung
  • Iga Mayola Pisacha Universitas Aisyah Pringsewu, Lampung
  • Vicko Suswidiantoro Universitas Aisyah Pringsewu, Lampung
  • Diah Kartika Putri Universitas Aisyah Pringsewu, Lampung

Keywords:

Beras kencur herbal drink, saccharin, Gadingrejo Market, U-HPLC

Abstract

Artificial sweeteners such as saccharin and sodium cyclamate are commonly used in foods and beverages; however, their usage must not exceed the limits established by the Indonesian National Agency of Drug and Food Control (BPOM) Regulation No. 29 of 2021, which sets maximum levels at 120 mg/kg for saccharin and 11 mg/kg for sodium cyclamate. Objective: This study aimed to identify and determine the levels of saccharin and sodium cyclamate in beras kencur herbal drinks with the sample code (JBKS) sold at Gadingrejo Market.Methods: The identification of saccharin was conducted using a qualitative test with resorcinol reagent, while sodium cyclamate was identified through a precipitation test. Quantitative analysis of saccharin and sodium cyclamate levels was performed using the Ultra High Performance Liquid Chromatography (UHPLC) method at a wavelength of 220 nm, with a mobile phase of methanol HPLC grade: aquabidest (80:20), stationary phase C18 octadecyl silica column, flow rate of 1 mL/min, retention time of 3 minutes, and injection volume of 20 µL. Results: The findings revealed that among seven beras kencur samples, one sample tested positive for saccharin, indicated by a green fluorescence color change. The quantitative analysis showed that the saccharin concentration in sample code JBKS 4 was 51,957mg in 3 servings a day. Conclusion: The saccharin level found in the sample exceeded the regulatory threshold, indicating that the beras kencur herbal drink is unsafe for consumption.

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Published

2026-08-29

How to Cite

Malik, M. Z., Pisacha, I. M., Suswidiantoro, V., & Putri, D. K. (2026). Analysis of Artificial Sweetener Levels in Beras Kencur Herbal Drinks Marketed at Gading Rejo Market Using U-HPLC. JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI), 5(2), 469–478. Retrieved from https://jurnal.undhirabali.ac.id/index.php/jakasakti/article/view/4910

Issue

Vol. 5 No. 2 (2026): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)

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Articles

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.