Radical Scavenging Activity of Ethanolic and n-Hexane Leaf Extracts of Physalis angulata L. Using The DPPH Assay

Authors

  • I Made Wisnu Adhi Putra Program Studi Biologi, Universitas Dhyana Pura, Bali
  • Ni Putu Widya Astuti Program Studi Sarjana Kedokteran, Universitas Dhyana Pura, Bali

DOI:

https://doi.org/10.36002/js.v5i1.5770

Keywords:

Antioksidan, DPPH, Ciplukan, Ekstraksi, Pelarut

Abstract

Physalis angulata L. leaves are known to have potential as a natural source of antioxidants, yet the effectiveness of extraction is highly dependent on the type of solvent used. This study aimed to compare the effects of polar solvent (ethanol) and non-polar solvent (n-hexane) on the phytochemical profile and antioxidant activity of P. angulata leaf extracts. The research methods included sample preparation of dried leaf powder, maceration extraction using ethanol and n-hexane, qualitative phytochemical screening to detect phenolics, flavonoids, terpenoids, saponins, and alkaloids, as well as antioxidant activity testing using the DPPH method to determine IC50 values. The results showed that the ethanol extract successfully detected all tested compound groups (phenolics, terpenoids, flavonoids, saponins, and alkaloids), while the n-hexane extract only detected phenolics and terpenoids. The antioxidant activity of the ethanol extract was classified as strong with an IC50 value of 87.098 ± 1.195 ppm, significantly better than the n-hexane extract which was very weak with an IC50 of 379.242 ± 7.452 ppm, and this difference was statistically significant (p < 0.0001). This study provides an important contribution by offering scientific recommendations that ethanol is the most effective solvent for extracting antioxidant compounds from P. angulata leaves, thereby serving as a reference for the development of health products and functional foods based on natural antioxidants.

References

Aviah, A. A., Rahmawati, R., & Syarif, S. (2024). Aktivitas antioksidan ekstrak etanol batang ciplukan (Physalis angulata L.) menggunakan DPPH. Makassar Pharmaceutical Science Journal (MPSJ), 1(4), 373–381. https://doi.org/10.33096/mpsj.v1i4.112

Dew, I. S., Saptawati, T., & Rachma, F. A. (2021). Skrining fitokimia ekstrak etanol kulit dan biji terong belanda (Solanum betaceum Cav.). Prosiding Seminar Nasional Unimus, 4(0), 1210–1218.

Farida, M., Azhari, S., Darsa, D. D., & Mahmudi, M. (2025). Skrining fitokimia dan uji aktivitas antioksidan ekstrak etanol daun ciplukan (Physalis angulata L) di kawasan manifestasi Geothermal Seulawah Agam. Journal of Pharmaceutical and Sciences, 8(1), 571–580. https://doi.org/10.36490/journal-jps.com.v8i1.826

Firawati, F., & Pratama, M. I. (2018). Isolasi dan identifikasi senyawa saponin daun bungkus (Smilax rotundifolia) menggunakan metode spektrofotometri ultraviolet. Jurnal Farmasi UIN Alauddin Makassar, 6(2), 115–121. https://doi.org/10.24252/jfuinam.v6i2.6757

Hantoro, B. M., & Kustiawan, P. M. (2024). Antioxidant activity of bee pollen Tetragonula testacetarsis from East Kalimantan. JOPS: Journal of Pharmacy and Science, 8(1), 133–140.

Happy, T. A., Septiani, L., Graharti, R., & Andrifianie, F. (2025). The potential of secondary metabolite compounds from indonesian medicinal plants as antioxidant agents: A literature review. Medical Profession Journal of Lampung, 15(3), 619–623. https://doi.org/10.53089/medula.v15i3.1825

Mahmudi, M., Azhari, S., Apriani, A., Rinaldi, R., & Uliani, R. (2026). Analisis kadar total fenolik ekstrak etanol daun ciplukan (Physalis angulata L.) dengan metode partisi bertingkat di kawasan geothermal dan non-geothermal. Journal of Pharmaceutical and Sciences, 9(2), 1643–1650. https://doi.org/10.36490/journal-jps.com.v9i2.1262

Maulana, F. S., Allaily, & Daud, M. (2025). Antioxidant activity test and phytochemical screening of ciplukan extract (physalis angulata) as an alternative feed additive in poultry. IOP Conference Series: Earth and Environmental Science, 1476(1), 012081. https://doi.org/10.1088/1755-1315/1476/1/012081

Mohammed, E. A., Abdalla, I. G., Alfawaz, M. A., Mohammed, M. A., Al Maiman, S. A., Osman, M. A., Yagoub, A. E. A., & Hassan, A. B. (2022). Effects of extraction solvents on the total phenolic content, total flavonoid content, and antioxidant activity in the aerial part of root vegetables. Agriculture, 12(11), 1820. https://doi.org/10.3390/agriculture12111820

Myint, A. A., Wulandari, S., Cao, R., Choi, J., Jaeryeong, J., & Kim, J. (2025). Unveiling multifunctional bioactive phytochemicals and antioxidants in tangerine pomace for value-added applications. Food Chemistry, 493, 145781. https://doi.org/10.1016/j.foodchem.2025.145781

Novitasari, A., Rohmawaty, E., & Rosdianto, A. M. (2024). Physalis angulata Linn. As a medicinal plant (Review). Biomedical Reports, 20(3), 47. https://doi.org/10.3892/br.2024.1735

Park, J., Lee, J., Cho, C., Lee, D. K., Song, W., Choi, K., Choi, S. Y., & Yang, H. (2025). Mass spectral data of primary and secondary metabolites changes in medicinal plants by solvent polarity. Scientific Data, 12(1), 893. https://doi.org/10.1038/s41597-025-05262-8

Pindan, N. P., Daniel -, Saleh, C., & Magdaleni, A. R. (2021). Phytochemical test and antioxidant activity test of n-hexane fraction extract, ethyl acetate and remained ethanol from leaf of sungkai (Peronema canescens Jack.) using DPPH method. JURNAL ATOMIK, 6(1), 22–27.

Putra, I. M. W. A., Fakhrudin, N., Kusumawati, I. G. A. W., Nurrochmad, A., & Wahyuono, S. (2022). Antioxidant properties of extract combination of Coccinia grandis and Blumea balsamifera: An in vitro synergistic effect. Journal of Herbmed Pharmacology, 11(1), 55–62. https://doi.org/10.34172/jhp.2022.06

Riskiana, N. P. Y. C., & Vifta, R. L. (2021). Kajian pengaruh pelarut terhadap aktivitas antioksidan alga coklat genus sargassum dengan metode DPPH. Journal of Holistics and Health Sciences, 3(2), 201–213. https://doi.org/10.35473/jhhs.v3i2.80

Rohadi, I., Erwin, E., & Aziz, A. (2024). Uji fitokimia dan uji toksisitas ekstrak etanol kulit batang kratom (Mitragyna speciosa Korth.) dengan metode brine shrimp lethality test (BSLT). PROSIDING SEMINAR NASIONAL KIMIA, 2(2), 53–58.

Sukma, M., Nurlansi, & Nasrudin. (2022). Total fenolik dan aktivitas antioksidan seduhan kulit batang soni (Dillenia serrata Thunb). Sains: Jurnal Kimia Dan Pendidikan Kimia, 11(1), 27–34.

Tsaturyan, A., Sahakyan, L., Hayrapetyan, L., Minasyan, E., Chakhoyan, A., Mirzoyan, V., Hovhannisyan, G., Khachatryan, H., & Hayrapetyan, S. (2025). Effect of solvent polarity on the extraction of bioactive compounds from Heracleum persicum fruits. Pharmacia, 72, 1–9. https://doi.org/10.3897/pharmacia.72.e168925

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Published

2026-07-21

How to Cite

Putra, I. M. W. A., & Astuti, N. P. W. (2026). Radical Scavenging Activity of Ethanolic and n-Hexane Leaf Extracts of Physalis angulata L. Using The DPPH Assay. JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI), 5(1). https://doi.org/10.36002/js.v5i1.5770

Issue

Vol. 5 No. 1 (2026): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)

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Articles

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.