Pengoptimalan Menggunakan Central Composite Design (CCD) untuk Depolimerisasi Kitosan secara Degradasi Oksidatif dengan NaNO2
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Dhian Eka Wijaya
Edwin Permana
Martina Asti Rahayu
Nur Ahniyanti Rasyid
Ali Nurdin Hidayat
Abstract
Optimalisasi proses depolimerisasi kitosan melalui degradasi oksidatif dilakukan
menggunakan natrium nitrit (NaNO2) sebagai agen oksidator untuk menghasilkan Low
Molecular Weight Chitosan (LMWC). Metode eksperimen menggunakan desain eksperimen
Central Composite Design (CCD) berbasis Response Surface Methodology (RSM) untuk
mengidentifikasi pengaruh berbagai faktor proses terhadap tingkat depolimerisasi kitosan.
Depolimerisasi kitosan dilakukan untuk pengembangan manfaatnya melalui proses
derivatisasi untuk memperluas jangkauan aplikasinya. LMWC hasil depolimerisasi memiliki
sifat mudah larut dalam air/larutan berair dengan pH netral dan memiliki viskositas yang
lebih rendah dalam kondisi fisiologis sehingga lebih mudah diaplikasikan di berbagai bidang,
seperti biomedis dan pertanian. Faktor-faktor yang dimasukkan dalam eksperimen adalah
suhu reaksi (A), konsentrasi NaNO2, dan waktu reaksi. Analisis statistika menunjukkan data
eksperimen mengikuti model linier dengan nilai koefisien determinasi (R2) sebesar 0,9219.
Nilai optimal variabel independen pada 50 C; 0,3 M NaNO2, dan 1,5 jam. Pengukuran Mv
pada kondisi ini memiliki ketepatan 94.35% dengan penurunan bobot molekul sebesar
83.68%.
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