Efek Ekstrak Teh Hijau (Camellia Sinensis) terhadap Jumlah Sel Leydig, Sel Spermatid dan Diameter Tubulus Seminiferus pada Mencit Jantan

Abstract

ABSTRAK
Boraks merupakan salah satu sumber radikal bebas yang dapat menyebabkan stres oksidatif. Teh hijau mengandung epigallocatechin gallat yang dapat mengikat superoksida dan radikal hidroksil, serta meningkatkan CAT, SOD, dan GSH-PX sehingga dapat mengurangi kerusakan sel Leydig, sel spermatid, dan tubulus seminiferus akibat stres oksidatif. Penelitian ini bertujuan untuk mengetahui efek pemberian ekstrak teh hijau terhadap jumlah sel Leydig, jumlah sel spermatid, dan diameter tubulus seminiferus yang diinduksi boraks. Penelitian eksperimental posttest only control group design, subjek uji 35 ekor mencit jantan strain Balb/C dibagi secara acak dalam 6 kelompok. Perlakuan dilakukan selama 35 hari. Hari ke-36 dilakukan pengambilan jaringan testis untuk dibuat preparat pengecatan HE dan diamati dengan perbesaran 400x sebanyak 3 lapang pandang. Jumlah sel Leydig, jumlah sel spermatid, dan diameter tubulus seminiferus dihitung dengan aplikasi ImageJ dan dihitung reratanya. Data dianalisis dengan uji One Way Anova dilanjutkan uji Post Hoc LSD. Hasil uji One Way Anova 0,001 pada jumlah sel Leydig, 0,000 pada jumlah sel spermatid, dan 0,000 pada diameter tubulus seminiferus. Ketiganya menunjukkan hasil yang signifikan (p<0,05). Kesimpulan dari penelitian ini adalah ekstrak teh hijau dapat meningkatkan jumlah sel Leydig, jumlah sel spermatid, dan diameter tubulus seminiferus pada mencit jantan yang diinduksi boraks.
Kata Kunci: Ekstrak teh hijau, sel Leydig, sel spermatid, diameter tubulus seminiferus, boraks.
ABSTRACT
Borax is one of free radical resources that can cause oxidative stress. Green tea contains epigallocatechin gallat that can scavenge superoxide and hidroxyl radical, and increase the number of CAT, SOD, and GSH-PX leading to reduced Leydig cell, spermatid cell, and seminiferous tubules damage. This study was aimed to determine effect of green tea extract on the number of Leydig cell and spermatid cell, seminiferous tubules diameter in boric acid testicular toxicity in mice. This experimental study using post test only control group design. 35 BALB/c male mice, divided randomly into six groups. Treatments were given during 35 days. On day 36, testicular tissues were taken for H&E stained histological preparation and observed under microscope with 400x magnification. The number of Leydig and spermatid cell, and seminiferous tubules diameter was counted with an application named ImageJ to 3 field of view and counted the mean. Data were analized using One Way Anova and continued with Post Hoc tes LSD. The result of One Way Anova test was 0,001 for number of Leydig cell, 0,000 for number of spermatid cell, and 0,000 for seminiferous tubules diameter. Three of them were <0,05 showing that green tea extract could increase the number of Leydig cell and spermatid cell, seminiferous tubules diameter. The conclusion is green tea extract could increase the number of Leydig cell and spermatid cell, and seminiferous tubules diameter in boric acid induced testicular toxicity in mice.
Keywords: green tea extract, Leydig cell, spermatid cell, seminiferous tubules diameter, borax