Krim Ekstrak Daun Binahong (Anredera condifolia (ten) steenis) Mempercepat Penyembuhan Luka Insisi Tikus Wistar Jantan
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. Liliawanti
Ferbian Milas Siswanto
Abstract
Tujuan penelitian ini adalah untuk membuktikan bahwa krim daun binahong dapat meningkatkan jumlah neovascularisasi, jumlah fibroblas dan mempercepat penutupan epitel pada tikus (Rattus norvegicus) jantan yang luka. Penelitian ini merupakan penelitian eksperimental, dengan post test only control group design menggunakan 28 ekor tikus jantan wistar yang dilukai. Pemilihan sampel dilakukan secara random, terbagi dalam 4 kelompok, yaitu 2 kelompok kontrol yang diberi krim plasebo untuk 4 dan 8 hari dan 2 kelompok perlakuan yang diberi krim daun binahong untuk 4 dan 8 hari. Setelah itu dilakukan pengambilan kulit tikus pada tempat yang luka untuk dilakukan pewarnaan hematoxylin eosin dan dilihat hasil histopatologinya untuk melihat neovaskularisasi, fibroblas dan penutupan epitelnya. Hasil menunjukkan rerata neovaskularisasi pada kelompok (P0) adalah 10,71±1,799, pada kelompok (P1) adalah 14,43±0,976, pada kelompok (P2) adalah 9,71±3,817, dan pada kelompok (P3) adalah 7,29±1,496 (p<0,01). Hasil penelitian juga menunjukkan jumlah sel fibroblas pada kelompok (P0) adalah 117,43±14,022, pada kelompoK (P1) adalah 104,71±27,415, pada kelompok (P2) adalah 81,57±30,010, dan pada kelompok (P3) adalah 51,29±13,238 (p<0,01). Selain itu, hasil penelitian ini juga menunjukkan celah epitel pada kelompok (P0) adalah 4575,86 ± 1420,89 μm, pada kelompok (P1) adalah 1571,78 ± 1082,78 μm, pada kelompok (P2) adalah 1903,83 ± 1252,96 μm, dan pada kelompok (P3) adalah 302,19 ± 799,54 μm (p<0,01). Simpulan: pemberian krim daun binahong pada luka dapat meningkatkan jumlah neovaskularisasi, tidak meningkatkan jumlah fibroblas dan tidak meningkatkan penutupan epitel.
Kata kunci: Anredera condifolia (ten) steenis, neovaskularisasi, fibroblas, penutupan epitel, luka.
ABSTRACT
The purpose of this study was to prove that the cream leaves binahong can increase the number of neovascularisasi, the number of fibroblasts and accelerate the closure of epithelium in rats (Rattus norvegicus) males are injured. This research is experimental research, with the post test only control group design using 28 male wistar rats which were wounded. The selection of the sample is done on a random, divided into 4 groups, i.e. 2 the control group who were given a placebo cream for 4 and 8 days and two groups who were given treatment cream leaves binahong to 4 and 8 days. After that is done taking the skin of mice at the cuts to do the coloring of hematoxylin eosin and seen the results of histopatologi to see the choroidal, fibroblasts and closing epitel. The results show average choroidal Group (P0) is ± 10,71 1,799, group (P1) is ± 14,43 0,976, group (P2) are the 9,71 ± 3,817, and group (P3) is ± 7,29 1,496 (p < 0.01). Research results also showed the number of fibroblasts in cell groups (P0) was 117,43 ± 14,022, at kelompo (P1) was 104,71 ± 27,415, group (P2) was 81,57 ± 30,010, and the Group (P3) was 51,29 ± 13,238 (p < 0.01). In addition, the results of the study also showed a crack group of epithelial (P0) was 4575.86 ± 1420.89 μm, group (P1) was 1571.78 ± 1082.78 μm,the Group (P2) was 1903.83 ± 1252.96 μm, and the Group (P3) was 302.19 ± 799.54 μm (p < 0.01). Conclusion: The granting of leaves of binahong cream on wound can increase the amount of choroidal, decreasing the number of fibroblasts and decreasing the closure of the epithelium.
Keywords: Anredera condifolia (ten) steenis, choroidal, fibroblasts, epithelial, wound closure at
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