Efek Ekstrak Teh Hijau (Camellia Sinensis) terhadap Jumlah Sel Leydig pada Mencit Jantan Strain Balb/C yang Diinduksi Boraks

Dini Millatul Azka, Ulfah Dian Indrayani, Meidona Nurul Milla

Abstract


ABSTRAK
Boraks merupakan salah satu sumber radikal bebas yang dapat menyebabkan stres oksidatif. Teh hijau mengandung epigallocatechin gallat yang dapat mengikat superoksida dan radikal hidroksil, serta meningkatkan CAT, SOD, dan GSH-PX sehingga dapat mengurangi kerusakan sel Leydig akibat stres oksidatif. Penelitian ini bertujuan untuk mengetahui efek pemberian ekstrak teh hijau terhadap sel Leydig yang diinduksi boraks. Penelitian eksperimental posttest only control group design, subjek uji 35 ekor mencit jantan strain Balb/C dibagi secara acak dalam 6 kelompok. Perlakuan dilakukan selama 35 hari. Hari ke-36 dilakukan pengambilan jaringan testis untuk dibuat preparat pengecatan HE dan diamati dengan perbesaran 400x sebanyak 3 lapang pandang. Jumlah sel Leydig dihitung dengan aplikasi ImageJ dan dihitung reratanya. Data dianalisis dengan uji One Way Anova dilanjutkan uji Post Hoc LSD. Rerata jumlah sel Leydig K1 24,44±2,47; K2 18,33±4,84; K3 33,11±5,14; K4 26,28±6,42; K5 31,89±5,05; dan K6 25,61±6,87. Hasil uji One Way Anova 0,001 (p<0,05) menunjukkan hasil yang signifikan. Hasil uji Post Hoc LSD menunjukkan terdapat perbedaan bermakna antara: K1 dengan K2; K2 dengan K4, K5, K6; antara K5 dengan K6 tidak berbeda bermakna. Kesimpulan dari penelitian ini adalah ekstrak teh hijau dapat meningkatkan jumlah sel Leydig pada mencit jantan yang diinduksi boraks.
Kata Kunci: Ekstrak teh hijau, sel Leydig, boraks.
ABSTRACT
Borax is one of free radical resources that can cause oxidative stress. Green tea contains epigallocatechin gallat that can scavenge superoxide and hydroxyl radical, and increase the number of CAT, SOD, and GSH-PX leading to reduced Leydig cell damage. This study was aimed to determine effect of green tea extract on the number of Leydig cell in boric acid testicular toxicity in mice. This experimental study using posttest only control group design. 35 BALB/c male mice, divided randomly into 6 groups. The treatments were given during 35 days. On day 36, the testicular tissues were taken for H&E stained histological preparation and observed under microscope with 400x magnification. The number of Leydig cell was counted with an application named ImageJ to 3 field of view and counted the mean. Data were analyzed using One Way Anova and continued with Post Hoc tes LSD. The mean of Leydig cell number of K1, K2, K3, K4, K5, K6 were 24,44±2,47; 18,33±4,84; 33,11±5,14; 26,28±6,42; 31,89±5,05; 25,61±6,87 respectively. The result of One Way Anova test was 0,001 (p<0,05) showing that green tea extract could increase the number of Leydig cell. The result of Post Hoc test with LSD showing that there was a significantly different between: K1 and K2; K2 and K4, K5, K6. The conclusion is green tea extract could increase the number of Leydig cell in boric acid induced testicular toxicity in mice.
Keywords: green tea extract, Leydig cell, borax

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